Imaging on a Large Scale - Expansion Microscopy and Spatially Resolved Transcriptomics

Recent advances in science and technology point towards the “omics” world. Omics-related sciences aim to gather information about Xn biological molecules to characterize and quantify the entire pool of molecules. Not surprisingly, microscopy is evolving to incorporate omics-related technologies, with spatially resolved transcriptomics being the latest cutting-edge microscopy approach to “Omics”.

Figure 1 - Spatial Resolved Transcriptomics allows researchers to achieve an Atlas of gene expression in 2D or 3D tissues.

Concomitantly, there is an urge to gather super-resolved information to capture the detail at a single-molecule level with light microscopy imaging. Expansion Microscopy (ExM) is an imaging protocol that allows conventional light microscopes to see sub-diffraction limited (<200 nm) or densely packed details that previously could not be distinguished.

Both technologies, spatial transcriptomics and expansion microscopy need specific features in a microscope with the requirements being high-contrast imaging of large samples at high resolution and speed.

Read our poster to see why Andor Dragonfly is the ideal microscope solution for imaging spatial transcriptomics and expansion microscopy.

Multiplex Imaging

• https://andor.oxinst.com/learning/view/article/dragonfly-high-speed-confocal,-in-situ-sequencing-and-multiplex-imaging
• https://andor.oxinst.com/learning/view/article/revealing-the-spatial-regulation-of-the-transcriptome-using-split-fish

Expansion Microscopy

• https://andor.oxinst.com/learning/view/article/what-is-expansion-microscopy-getting-the-most-from-expanded-samples
• https://andor.oxinst.com/learning/view/article/dragonfly-the-ideal-confocal-system-for-expansion-microscopy
• https://andor.oxinst.com/learning/view/article/revealing-the-nanoscale-organisation-of-the-cell-using-expansion-microscopy-demo