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The game-changer in confocal microscopy. With the Andor Dragonfly, you can image at an unrivalled combination of speed, sensitivity, confocality and resolution beyond the diffraction limit. Now available in 200 series and 500 series.
NEW Fusion 2.0 provides a workflow from nm to mm in a single solution
The Dragonfly naturally benefits from Andor EMCCD and sCMOS technologies, with motorized optical zoom, the system delivers industry-leading signal to noise and image fidelity. Applications ranging from single molecule to live cell confocal, TIRFM to whole embryo and thick tissue imaging all benefit from the system’s speed and sensitivity. Dragonfly delivers real-time visualization for rapid specimen evaluation and ClearView-GPU™ deconvolution to maximize resolution and throughput.
The Dragonfly offers you a sharp insight into the world of your specimen by employing a novel multi-point confocal system, optimized for fast, flexible and sensitive scanning. It extends your sample’s longevity and significantly speeds up data acquisition.
As well as confocal, the system provides further extraordinary control of illumination by offering enhanced widefield and TIRF microscopy, extending the reach from nm to mm and from single molecules to cells, tissues, organoids and embryos.
Traditional Point Scanning Confocal
Dragonfly Confocal Imaging System
| Laser Range | Confocal Speed | Apeture | Camera Zoom | Illumination Zoom | Pinholes Ø |
| 400 - 800 nm | 400 fps | 22 mm | 1x | 1.5x | 2x | 1x | 2x | 4x | 6x | 40 µm | 25 µm |
Dragonfly captures in 16-bit dynamic range, therefore can obtain both low and high signal detail within a single capture.
Dragonfly is now available in a more compact package, the 200 series, suitable for both inverted and upright microscopes.
The 200 series offers many of the key features that make Dragonfly performance exceptional: Confocal speeds of up to 400 fps; Borealis™ enhanced illumination for a large and flat field of view; ClearView-GPU™ deconvolution; dual-camera acquisition; choice of optical zoom and pinhole size.
For those who might be considering a new or additional confocal, but looking for something that pushes new imaging boundaries. The Dragonfly 200 offers a price-point comparable to traditional point scanning technology.
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Dragonfly 500 offers the full range of imaging modalities and complementary features available on this innovative platform.
Choose this model for a fully scalable imaging range from; sub-cellular protein targets at the cell membrane using TIRF, or deeper in the cell with SRRF-Stream and dSTORM super-resolution; to large scale tissue structure or whole organism anatomy.
Dragonfly 500 should be considered by those whose research projects, or variety of users, cover a wide range of imaging requirements; and for whom the ability to apply several pertinent techniques to the same sample offers a powerful tool for discovery.
Techniques for which you would purchase three independent products, Dragonfly 500 offers in a single cost-effective solution.
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The combination of Andor’s Dragonfly and Fusion software provide unparalleled access to super-resolution techniques to image beyond the diffraction limit of optical microscopy. Expand your research from single molecules to whole organisms. All on one system! Choose from our latest camera-based super-resolution technique, SRRF-Stream; iterative ClearView-GPU™ deconvolution or the localisation technique known as dSTORM. Each delivers a different and unique view into the nanometer world. Choose your technique according to your experimental needs.
Deconvolution is an invaluable tool for revealing the subtle details of a 3D data set that might otherwise go unnoticed, even in a good quality confocal stack. Break the resolution limit and attain a lateral resolution down to 140nm and axial resolution to 250nm.
ClearView-GPU™ is an integral part of the system to enhance the finest features of your image. Up to 50x faster than CPU methods, apply ClearView while acquiring images for immediate visualisation and comparison with the original data!
Use deconvolution for the highest 3D resolution, especially powerful as the only super-resolution tool that can be applied to high-speed imaging such as live-cell and multi-dimensional imaging.
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Developed in close collaboration with Dr Ricardo Henriques, University College London (UCL), SRRF-Stream offers real-time camera-based super-resolution for live cell imaging across large fields of view, such as those offered by Dragonfly.
SRRF-Stream can be applied to regular fluorescence probes down to a resolution of 50nm and does not require any special sample preparation. Consequently SRRF-Stream can be used on live samples, as well as fixed, and can capture up to 1 frame per second (512x512) ideal for the likes of cytoskeletal dynamics and organelle structure.
Depth colour coded projection of neurons in a 500um thick spinal cord prepared using the RapidClear method (Sunjin Labs). Volume captured to 100um deep. Zoomed region demonstrates increased resolution by the clarity of dendritic spines.
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Direct Stochastic Optical Reconstruction Microscopy, or dSTORM, is a single molecule localisation technique that requires high illumination power to drive fluorophores into a dark state. By collecting a large number of frames of different fluorophores within a region, a high-resolution image can be built up.
The Dragonfly 500 series provides the high illumination powers and an optional 3D lens to perform dSTORM acquisitions in 2D and 3D. The dynamic nature of post-processing algorithm developments means that, rather than restrict analysis to Andor software, users are free to select any of their preferred platforms for analysis. Choose to use capture dSTORM image sets when you require resolutions down 20nm.
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The Dragonfly naturally benefits from Andor EMCCD and sCMOS technologies, with motorized optical zoom, the system delivers industry-leading signal to noise and image fidelity. Applications ranging from single molecule to live cell confocal, TIRFM to whole embryo and thick tissue imaging all benefit from the system’s speed and sensitivity. Dragonfly delivers real-time visualization for rapid specimen evaluation and GPU-accelerated deconvolution to maximize resolution and throughput.
Discover how Dragonfly excels in:
Fusion Stitcher was developed to handle the large multi-dimensional image files Dragonfly generates. Start stitching whilst your montage is being captured, so saving valuable time. Stitch faster in 3D than other solutions can handle in 2D.
We offer the perfect solution for developmental models such as Zebrafish and C. elegans or mapping protein localisation in large areas of tissue such as brain and kidney. The speed of Dragonfly combined with the integrated Fusion Stitcher will save days of valuable time to you can submit that paper ahead of the game!
Large area super-resolution imaging with SRRF-Stream & Stitcher
Combine SRRF-Stream™ and Stitcher™ for the highest resolution imaging of target samples larger than the field of view of your objective.
Fusion is the result of over 100 person-years of shared imaging and development experience, making it easy to design an experiment, choose necessary devices and configure optimal data acquisition protocols. It simplifies the control of the Dragonfly system, translating imaging modes into software states and fluorophores into channels to help you build protocols in just a few mouse clicks.
Integrating deconvolution is simple, tasking the GPU with the associated calculations so that acquisition continues unaffected: a big time saver! Results are delivered with your data, all in one software package. Real-time 3D visualization, split channel view and MIP are fully configurable during acquisition to let you view the data in a mode which best suits your experiment.
Real-Time Rendering
Real-time rendering in Fusion allows for an on-going verification of the quality of your experiment and the status of your sample.
Having a near-instantaneous feedback on the 3D properties of the specimen is critical for evaluation of experimental parameters including signal to noise, illumination power and sample viability, its position in 3D and in relation to its cellular or tissue neighbours. Render quality and speed can be rapidly adjusted following any change in your experiment and can be easily accessed in the Fusion software control panel.
Contact our Software SpecialistsProtocol Manager
Protocol manager puts you in control of all the tools necessary to create acquisition sequences. It will guide you through the necessary steps of your imaging experiment to produce the most insightful and data-laden image stack.
Add channels from the desired imaging mode and tune the Protocol to your needs, before saving for re-use, per Windows user, along with channel settings and imaging modes. With Fusion software you can plan your experiment in advance, placing the system in an advanced state of readiness, able to begin imaging as soon as you give the go command.
Contact our Software SpecialistsImage Processing
Fusion’s ClearView-GPU™ deconvolution processing is up to 50x faster than CPU-based methods. There’s no longer a reason not to deconvolve every image and bring out the subtle details that otherwise go unnoticed, even in a good quality confocal stack.
Fusion provides automated tools to combine neighbouring images into large area datasets in 2D and 3D. This is often known as tiling, or montage. Dragonfly’s large field of view and illumination quality provided by Borealis, combined with Fusion’s image processing tools allow large samples to be captured in exquisite detail.
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Central to the Dragonfly concept is the goal of maximizing throughput, which is achieved by optimizing both imaging performance and data flow. Fusion and Imaris provide seamless transitions from imaging to deconvolution, visualization to analysis. As a result you spend more time collecting high quality data and less time pre-processing and transferring between systems.
Speed Hypothesis Testing
Hypothesis testing is a fundamental part of the scientific method. The familiar cycle of data creation and evaluation are delivered by the Dragonfly workflow, supporting demanding experimental regimes. Extended observations, large fields of view and high quality data provide the input for powerful statistical analysis and data mining.
All the necessary functionality for data management, visualization, analysis, segmentation and interpretation of 3D and 4D microscopy datasets with Imaris
Visualize Complex Data
Welcome to a new era of data interpretation, plotting and presentation. ImarisVantage enables you to compare and contrast experimental groups by visualizing your image data in five dimensions as uni- or multi-variate scatterplots. Along with the use of box + whisker plots Vantage will help you interpret intrinsically complex and dynamic phenomena.
ImarisVantage allows researchers to dissect their multi-dimensional, multi-object images by creating a series of fully customizable plots for better understanding of hidden relationships and associations between calculated measurements, objects or groups of objects.
Contact our applications specialistsTrace Filaments
FilamentTracer is the most advanced software product for the automatic detection of neurons (dendritic trees, axons and spines), microtubules, and other filament-like structures in 2D, 3D and 4D.
When combined with ImarisTrack, detection of temporal changes in length and volume of developing spines and dendrites will help researchers understand alterations caused by developmental and environmental changes.
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Analyse and Understand Cell Fate
ImarisLineage is the new module which builds on the functionality of the powerful and widely used ImarisTrack. ImarisLineage provides tools for accurate automated or manual tracking of dividing cells in 2D/3D time series.
ImarisLineage enables creation of an interactive lineage tree where the cell fate can be traced from one progenitor cell to the final generation.
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The Andor Learning Center hosts a wide range of tutorial videos, technical articles and webinars to guide you through the range of products for all your imaging needs. We have provided some links below which will get you started on some of our most recent uploads.
Dragonfly allows us to perform multi-colour (DAPI-, GFP-, RFP- and Far-red channels) 3D imaging with continuously high-resolution (confocal)-wide view mode and super-resolution (SRRF)-magnified view mode. From practical experience, I can say it has absolutely incredible features!
Dr Kazuaki Yoshioka, Kanazawa University Graduate School of Medical Science, Japan
Dragonfly 200, as it offered significant upgrades in performance and ability to customize the system to meet the needs of the laboratory. The level of service I receive from Andor is above that of other suppliers I have dealt with in the past. Andor has helped me drive my work forward to evolve both my academic research and the treatment options for critically ill patients.
Thomas Diacovo, MD, Professor of Pediatrics and Pharmacology, University of Pittsburgh
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