Photostimulation and Optogenetics Solutions

Photostimulation and Optogenetics enables precise optical manipulation of complex biological networks. More researchers are exploiting these techniques in fields as diverse as neural mapping, visual regeneration and vascular hypertension. Optogenetics allows observations that cannot be determined by other means at the organ, tissue and cellular levels.

NEW MicroPoint 4 - tuneable pulsed laser system for targeted ablation, bleaching and uncaging
Mosaic - digital micro mirror-based solution for real-time multi-region illumination and optogenetics

MicroPoint 4 and Mosaic 3 for Photostimulation and Optogenetics

MicroPoint 4

Precise Delivery of Laser-based Illumination

DNA damage - cancer research
Ablation - Damage and repair studies
FRAP and PA - cell dynamics
Uncaging - signaling

Specifications

Mosaic 3

Real-time, Multi-Region Illumination

Optophysiology/optogenetics
FRAP and PA - cell dynamics
Uncaging and signaling studies
Selective region imaging

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Matching Application Requirements

Illumination Power Required	Type of Experiment	Mosaic	Micropoint	Illumination Requirement	Example Applications
	Ablation Studies			Pulsed	Axotomy. Cut microtubules or cytoskeleton. Wound healing assays
	DNA Damage			Pulsed/CW	DNA damage/repair, gene expression, developmental and cancer studies
	Uncaging			Pulsed/CW	Determine function of biomolecules within the cell and/or organism context
	FRAP/FLIP/FRET			CW	Observe protein dynamics, fusion and binding events. Cell compartmentalisation and localization
	Photoswitching/Photoconversion			CW	Study the function of proteins and molecules within complex pathways
	Phenotype Marking			CW/LED	Simultaneous multi-region photoactivation. Simultaneous photoactivation for identification and sorting
	User Defined Complex Regions of Illumination			CW/LED	Define complex regions of interest: target specific cell structures, groups of cells or tissues
	Optophysiology and Optogenetics			CW/LED	Cell & Neuronal signalling, opsin controlled secondary messengers e.g. Ca2+. Optically controlled protein switching. Signalling cascades. Combine with electrophysiology

Key:

- Not Suitable

- Suitable for Some Applications

- Partially Suitable

- Recommended

Notes

Pulsed - A pulsed dye laser provides a wide range of wavelengths and high energy at up to 20 pulses per second. While a solid state pulsed laser delivers higher peak power at higher repitition rates.

CW - MicroPoint can be used with CW (continuous wave) lasers for scanned FRAP and other high power density applications. Mosaic can be coupled with Andor's HLE for high power bleaching applications across the visible spectrum.

LED - LED light sources coupled with Mosaic provide illumination powers suitable for many optogenetic probes e.g. ChRh, light-induced dimerization systems and many others.

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