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Laser Engines for Microscopy

Andor's laser engine portfolio offers exceptional quality packed into highly flexible devices. With up to 10 lines from the UV to the NIR aiming to meet users' current and future needs. It accommodates a wide range of applications in life sciences from Super Resolution Microscopy to long-term, gentle live imaging, TIRF and photostimulation.

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Laser Engine Solutions for Life Science

High Power Laser Engine (HLE)

  • Up to 10 laser lines
  • Wavelength range 405-785 nm
  • 400 - 2500 mW range
  • 3 output ports
Specifications Request Pricing

Integrated Laser Engine (ILE)

  • Up to 8 laser lines
  • Wavelength range 405-785 nm
  • 50 – 150 mW range
  • 3 output ports
Specifications Request Pricing

Laser Engines Techniques Matrix

Technology HLE ILE
Confocal Microscopy √ √ √ √ √ √
Widefield √ √ √ √ √ √
SRRF-Stream √ √ √ √ √ √
Live Cell Imaging √ √ √ √ √ √
Borealis TIRF √ √ √ √ √
Spatial Transcriptomics √ √ √ √
Photostimulation (MicroPoint) √ √ √ √
Photostimulation (Mosaic) √ √ √ -
SMLM - DNA Paint √ √ √ -
SMLM – dSTORM √ √ √ -

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High Power Laser Engine

Industry-Leading Power, Flexibility, Classic Wavelengths

High Power Laser Engine
Laser Lines - Up to 10
Wavelength Range - 405-785nm
Output mode - Multimode
Multiport switch time - < 7
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Specifications

Integrated Laser Engine

Third Generation Multi-line Laser Source

High Power Laser Engine
Laser Lines - Up to 8
Wavelength Range - 405-785nm
Output mode - Single and/or multi
Multiport switch time - 3
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Specifications

Laser Engines for Microscopy Applications

Super-resolution (dSTORM)​

dSTORM - uses small organic dyes (such as AF647, Atto488) that can have 3 excitation sates. High laser powers are needed to achieve the dark state (required for dSTORM imaging).

  • The patented Borealis® optical configuration allows illumination zoom (iZoom) to be supported in Dragonfly 600 series. iZoom enables power density multiplication in reduced fields of illumination.
  • HLE raises the bar on power density for single molecule localization techniques such as dSTORM especially when combined with B-TIRF, delivering enough power to reach the dark state and to perform dSTORM experiments even without the need of iZoom.
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Photostimulation

Photostimulation uses light to activate compounds in cells, tissues or organisms. This non-invasive process addresses several biological questions such as mapping neuronal connections, timely tracking protein cells, etc. Andor manufactures two photo stimulation devices: Mosaic 3 (DMD based) and MicroPoint 4 (fast galvo scanning).

HLE can be used as a light source for Mosaic and MicroPoint. The ILE can be used as light source for MicroPoint.

Application examples:

  • Map gene expression, e.g. in live lymph node sections
  • Address the mechanisms of mitotic fidelity.
  • Study the effect of DNA damage.
  • Address the effects of wound healing and repair C. elegans.
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DNA-PAINT

In DNA-PAINT - Point Accumulation In Nanoscale Topography - the sample is labelled with antibodies, are conjugated with a short, single strand of DNA called docking strand that is not fluorescent. The imager strand solution binds stochastically to the docking strand.

  • HLE provides intense laser illumination to highlight binding events for high precision localization.
  • HLE in combination with B-TIRF down to 5-10 nm can be achieved.
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Spatial Transcriptomics

Imaging tissue is the target of spatial “omics” research, which aims to understand tissue architecture, its spatial organization and how this impacts the genetic and functional profile of individual cells in-situ. Large tissue volumes are imaged, and fast imaging is an essential requirement.

  • HLE and ILE deliver the spectral output for high performance imaging.
  • HLE and ILE deliver the necessary power high performance imaging omics imaging.
  • HLE delivers the next level of productivity due to high lasers powers combined with decreased image acquisition time.
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Live Cell Imaging

Live cell imaging requires low phototoxicity/ photobleaching. High sensitivity is essential to be able to use the minimum light in imaging. Tight laser light control is required to adjust the imaging light toward the minimum required in the experimental protocol. In many cases a high temporal resolution is required, examples are applications such as microtubule dynamics, or cilia beating.

  • Low light levels are achieved in ILE and HLE by using the neutral density (ND) filters in the excitation light path, delivering up to 1000X attenuation in the laser power reaching the sample.
  • Image tumour development or hours or days with ILE or HLE
  • Image dynamic events such as calcium signalling with fast confocal imaging (400 fps).
  • Capture all signals with short exposure times and highly sensitive detectors.
  • Image deeper into live thick brain tissue with NIR wavelengths
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