The sensitivity needed depends on the application – high sensitivity is not always required, while EMCCD is still essential for when sensitivity is a priority. Modern microscopy cameras are developed around providing a good field of view from a microscope with sensors generally being designed within this size. As with the other parameters, field of view cannot be viewed exclusively with camera speed also being required to allow effective operative at large fields of view. Imaging speeds listed for a camera are not so simple for determining the real-world speed of an imaging camera. Other factors come into play such as the sensitivity of the camera, the read-out mode and the background noise levels.
A number of applications are summarized against their main imaging requirements. Suitable camera types are then suggested for these based on their ability to fulfil these criteria.
Key learning objectives :
The relative sensitivity of different camera technologies
Comparisons of field of view and how this can be matched to the microscope
Camera speed on paper versus what is required for running cameras in high speed imaging applications
sCMOS Rolling and Global shutter operation for high speed imaging
Techniques to boost imaging speed and limitations to these approaches
Important criteria for common imaging applications, and suitable cameras