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Microscopy
The advent of fluorescence microscopy allowed the revelation of a whole new world previously hidden inside cells and tissues. Epifluorescence microscopes are extremely useful to visualise thin samples or tissue sections. Still, when samples are a bit thicker, the out of focus light will decrease significatively the Signal to Noise Ratio of the image. Because of reduced signal to noise, the structures of interest will be surrounded by “haze” becoming more difficult or nearly impossible to visualise. It became necessary to develop another type of imaging that could deal with the out-focus light, and that would allow optical sectioning of the sample. The confocal microscope was developed to address these issues.
Confocal microscopes discard the out of focus light using pinholes. Optical sectioning of the sample is achieved using the pinholes in which the in-focus light reaches the detector, while the out of focus light is discarded.
There are two types of confocal microscopes:
In this lesson, Dr Claudia Florindo, Microscopy Specialist, Andor, will explain what a confocal microscope is and discuss the differences between single-point confocal and multipoint confocal. By the end of this talk, we hope that you will have a clear understanding of confocal microscopy and it´s applications in life sciences.
Learning Objectives:
Questions Answered:
Date: January 2020
Author: Dr Claudia Florindo
Category: Webinar