Mosaic 3 - Photostimulation & Optogenetics Tool

Simultaneous Illumination of Multiple Regions of Interest in Real Time

Mosaic 3 is a patented instrument platform built around a MEMS Digital Mirror Device (DMD). The DMD comprises an array of individually controllable micro-mirrors that can be switched “on and off”. DMD arrays contain hundreds of thousands to millions of micro-mirrors allowing precise control of illumination in user defined patterns or across multiple regions. These properties have made mosaic an exceptionally useful and versatile tool for optogenetic and photostimulation experiments.

Features and Benefits of Mosaic 3

Unlimited flexibility in shape, size, complexity of illumination mask
Simultaneous illumination of multiple regions of interest
Precise illumination of areas of interest that protects target specimen
and fluorophore
Zero delta acquisition time for true digital excitation
Complementary illumination option enables on and off control in optogenetics studies
Longest lifetime and lowest maintenance with semiconductor device

Specifications

Applications & Techniques

Fluorescence Recovery after Photobleaching (FRAP)

FRAP is the basis for many experiments that seek to determine processes that may be hidden by background fluorescence levels such as protein turnover, diffusion rates and many more.

Fluorescence recovery shown for A. thaliana
Mosaic 3 is a unique solution for FRAP providing user-defined illumination, or simultaneous illumination of multiple regions.
Boost productivity and insights by capturing multiple measurements in a single experiment.

Uncaging

Mosaic 3 is ideal for experiments that require biomolecules to be uncaged and photoactivated into their active state.

Use with UV sources including arc lamps, LED and lasers to uncage biomolecules with precision.
Amazing flexibility with features like user configurable regions of interest and multiple regions.
Uncaging used to study role of calcium and mitogen-activated protein kinase–NF-κΒ pathway. Professor Mike White and Dr. Violaine Sée, and Dr. Dave Spiller

Photoactivation

Photoactivation can be used in many ways: study cell dynamics & signalling, intracellular organelle tracking, or for ion imaging or neurotransmission.

One example is in determining the mobility of histones within the nucleus, in response to agonists and relative to other nuclear features such as nucleoli or other compartments.
Mosaic 3 DMD technology provides exceptional control of illumination across the image area without any time delay of scanned illumination.

Mosaic 3 allows you to continuously illuminate an arbitrary mask in the imaging field. You simply can’t do this using laser-scanning microscopes, which never provide true simultaneous illumination. This important feature combined with ease of use and direct interfacing with software makes the Mosaic a unique and valuable tool for the optogenetic community.

Professor Harald Janovjak, Matthew Flinders Professor in Molecular Medical Bioengineering and Head of the Synthetic Physiology Laboratory at Flinders University

Learning Resources

Andor Virtual Camera – A Versatile Tool for Photostimulation Targeting

Photostimulation techniques (PS) are important tools for the study of materials and live biological specimens. Typically, PS uses targeted optical radiation to induce some kind ...

Uncovering the Membrane Mechanism of Cytokinesis Using Live Cell Imaging and Subcellular Optogenetic Tools

In this webinar, the following questions will be addressed: What are the membrane remodelling events that occur during cleavage furrow formation? What is SRRF-Stream? How c...

Optogenetics – Bringing the Inner Processes of the Brain into the Light

Optogenetics combines optics and genetic manipulation techniques to allow for very precise control of processes within cells using light. With an interconnected network of billi...

Andor High Power Mosaic Lasers – SSL-HP Series

The Andor Mosaic® high performance laser diode modules (SSL-HP) have been created specifically to address the needs of high-end applications requiring superior optical quali...

Electrophysiology Meets Optogenetics

Electrophysiology meets Optogenetics combining Axon pCLAMP™ with Andor Mosaic® Browse through the presentation slides below...

Using Optical Inhibition of Specific Neurons to Unravel the Complexities of the Brain

Scientists can learn about the functions of specific neurons in complex networks, like the brain using proteins that act as “photoswitches”. Optogenetics has proven ...

An overview of Optogenetics - Neurons and Beyond in neutral research

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