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BC43 WF - Benchtop Widefield Fluorescence Microscope

Cost Effective Widefield Fluorescence Microscopy

BC43 WF is a new addition to our benchtop microscope range, ideal for routine imaging or small budgets and with so much growth potential!

The system enables hassle-free, high-quality widefield fluorescence imaging, and is fully upgradable in the field to allow researchers to extend into confocal and super-resolution microscopy as requirements or budgets increase.

BC43 WF: Affordable and Upgradable.

BM42 Model also available. Andor's most affordable widefield microscope matching BC43 WF's performance and imaging quality (confocal upgrade not available).

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The Fully Upgradable Fluorescence Microscope You Can Trust

BC43 WF benchtop widefield fluorescence microscope is ideal for early-stage researchers whose needs will change over time. BC43 WF is feature-rich to accommodate a variety of experimental protocols in a small, easy to use package. When your needs exceed the widefield fluorescence and brightfield imaging capabilities of BC43 WF, you can simply expand and upgrade to confocal and super resolution.

Andor is the first microscope manufacturer to offer an IQ / OQ quality control programme which ensures that all BC43 microscopes operate within tightly controlled parameters, providing confidence that your data and experiments are reproducible in the future.

Click the hotspots below to explore the key features and benefits of Andor BC43.

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Widefield Fluorescence & Transmitted Light Microscopy All-In-One

Our sensitively priced entry model BM42 provides an upgrade path to widefield Super-resolution, and if your future needs require confocal imaging then the BC43 WF is ready for upgrade when you are. A Microscope that Grows with Your Needs...

All upgrades are performed onsite in your lab or facility with no need to return your system to a factory or service site.

Widefield Fluorescence Microscopy

  • The most used and routine fluorescence microscopy method.
  • Perfect for fixed samples such as sectioned tissue and monolayer cultures.
  • Incubation options add live-cell imaging capabilities for imaging cellular dynamics.

Add ClearView™ GPU digital confocal to remove sample blur, enhancing resolution and ensuring best quality images.

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Transmitted Light Microscopy

BC43 also delivers two different transmitted light options for amazing imaging flexibility:

  • Use Brightfield Mode to visualise the development of larger organisms whose structures naturally deliver high contrast images
  • For thinner samples and/or those with higher transparency, use Differential Phase Contrast (DPC) mode to draw out their structure

You can also mix and match modes like DPC and Widefield for even more imaging flexibility!

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High-Speed Fluorescence Imaging for Biologists

The BC43 Benchtop Microscope was developed in collaboration with leading biologists for use across life science applications and is designed to be part of a single room workflow. Andor's BC43 Widefield Fluorescence Microscope will bring an ease of use and flexibility to your research, whatever your sample, in ways previously only found in larger, more complex and expensive imaging systems.

Cancer

BC43 addresses the broad scales and types of imaging protocols and analysis related to cancer research. BC43 WF can handle subcellular/cellular live cell experiments and imaging tissue sections, with a confocal upgrade to BC43 CF extending to large tissue samples and organoids requiring deeper imaging.

  • High productivity imaging of tissue preparations and fixed cells
  • Follow cell dynamics (intercellular behaviour, migration and division)
  • Upgrade image quality and resolution with ClearView™ haze removal and/or super-resolution
  • Analyse movement, cell lineage, count cells, features and more...

Pancreatic ductal adenocarcinoma cells inside and on top of a 3D hydrogel. DNA is blue, lamin A/C is green, and tubulin is orange. Image credit: Sebastian Amos and Yu Suk Choi, University of Western Australia.

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Cell Biology

BC43 conquers the diverse range of protocols that Cell Biology experiments demand: from a simple 2D snapshot of a cell to understanding dynamic processes with time-lapse.

  • BC43 WF provides excellent illumination to shorten exposures for high-productivity imaging or fast live-cell dynamics.
  • The sensitive camera will facilitate imaging of low fluorescence signals.
  • Adding ClearView™ haze removal or Super-resolution will boost image clarity and resolution.
  • Adding hardware-based confocal will extend your imaging capabilities when you are ready.
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Core Facilities

Andor’s Benchtop Microscope is a compact, but fully featured system for routine imaging. It can be the imaging workhorse you are looking for.

Simplicity of use has been designed into BC43 to reduce training time and ongoing support of users, enabling user independence and improving facility efficiency.

Researchers will rapidly become competent in acquiring excellent images, and higher multi-dimensional experiments on their own.

  • Four excitation lines (405, 488, 561 & 640nm) covering most popular fluorophores.
  • Up to 5 imaging objectives (user friendly to exchange as required)
  • Adaptable to confocal and/or Super-resolution if your facility needs change.
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Developmental Biology

Cut through the challenges of your 3D world and capture images in perfect clarity - from the very first rounds of cell division to the fully developed organism.

Extract crisp 2D images or explore stunning 3D volumes in a fraction of the time you’re used to. Andor’s Benchtop Microscope delivers fast imaging of developing model organisms such as zebrafish and Drosophila.

  • Capture early rounds of cell division with sensitivity and speed.
  • Follow cell differentiation, morphogenesis and more.
  • Add ClearView™ haze removal or hardware-based confocal for more mature model organisms.
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Neuroscience

BC43 WF provides high-productivity imaging of brain tissue sections and high-resolution images of neuronal cultures.

Upgrade to confocal for thicker samples such as organoids, expanded tissue, and small model organisms such as zebrafish and C.elegans.

  • Gentle illumination and high sensitivity for live imaging.
  • High quality stitching for high-resolution neuronal morphology imaging.
  • Add super-resolution for fine detailed imaging of neuronal processes.
  • Dedicated image analysis tools with Imaris: automated process tracing, auto depth, spine detection and more…
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Tissue Imaging

Capture stunning 2D and 3D images from live tissue models to whole cleared-tissue preparation

Start withBC43 WF for 2D and thin 3D imaging, and upgrade BC43 CF to confocal to image deeper than widefield epifluorescence.​

  • Time-saving, large-view, high-resolution imaging of tissue.
  • Capture huge samples with tiled volume imaging and stitching.
  • Innovative analysis tools available with Imaris: machine learning object classification, spatial analysis, volume and morphology and many more.
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Powerful and Intuitive Acquisition Software

At the heart of BC43 is a software that is not only feature-rich and flexible to your needs, it is easy-to-use as well:

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Comprehensive Image Analysis Software with AI Tools

All Andor BC43 Benchtop Microscopes come with the introductory Imaris Quant package for users to:

Additional Imaris modules are also available including tools for tracking and reporting motility metrics, quantifying cells and their intracellular organelles, and tracing neurons for reporting their specific characteristics.

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Prof. Dr. Michael Kiebler

The BC43 WF works very well in our laboratory. As frequent users of conventional fluorescence microscopes, we were unsure if a benchtop model matched their quality given the optical performance our high magnification neuronal culture imaging demands. However, the BC43 WF not only met our high expectations, but it was much easier to use, resulting in many curious researchers that wanted to use the machine for their experiments.

Prof. Dr. Michael Kiebler, Department for Cell Biology, BioMedizinisches Centrum, Ludwig Maximilians Universitat Munchen.

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