The Fundamentals of Single Molecule Detection

Single Molecule Detection has become an important technique and may be used as a basis for super-resolution for enhanced resolution of intracellular components as well as tracking processes at the molecular level. It provides a route to additional insight into the cellular machinery that is otherwise hidden in more conventional “bulk” imaging methodologies.

In this first webinar of the Single Molecule Imaging series, Dr Justin Cooper provides an introduction to some of the main concepts within Single Molecule Detection. This includes topics such as: what is the point spread function, why is signal to noise important, what detector options are available and how we can detect, localise and track single molecules. Justin then discusses some of the wider range of applications that single molecule detection techniques may be used for.