Modelling Of Cellular Interactions In Mixed Murine Organoids

Cell competition is a mechanism of interaction that dictates cell selection based on differences in cellular fitness. We developed a model of mixed murine organoids and enteroid monolayers to study such complex cellular interactions in a mammalian system. We combined this model with (time-lapse) microscopy and transcriptome analysis to show that cell competition drives growth of cancer cells by active out-competition of wild-type cells through forced cell death and cell-state change in a JNK-dependent manner.