Microscopy School Lesson 2 – Transmitted Light Microscopy
Although recent advances have offered researchers many sophisticated imaging technologies, transmitted light microscopy techniques are still applicable to a wide variety of research areas. Transmitted light microscopy images are useful to analyse the morphological features of biological samples. Furthermore, transmitted light techniques also they deliver an “extra channel” that can provide context to the fluorescence stainings. Importantly, due to the very low energy use in transmitted light microscopy techniques, they are extremely adequate for live imaging experiments. Therefore, knowledge of transmitted light microscopy techniques; its differences and applications are a valuable tool for researchers.
In this seminar, Dr Claudia Florindo (Life Science Specialist, Andor Technology) will review the anatomy of the microscope and essential concepts in microscopy, such as numerical aperture and resolution. We will explain the bases of the differences between the different transmitted light techniques. Finally, to give a clear understanding between the optical requirement for the techniques and the resulting image, we will show images acquired with the different techniques.
Overall, we aim that students attending this class, can both understand what is transmitted light microscopy, and discover what the best technique for their application is.
Learning Objectives: 1. Recall the anatomy of the microscope. 2. Recognize different transmitted light microscopy techniques. 3. Choose the best-transmitted light microscopy technique for your application.
Questions Answered: - How does the different correction in objectives impact your image? - What is the NA (Numerical aperture)? - How can I have even illumination of my sample?