High Resolution Imaging of Zebrafish at Nano and mm Scale using Andor Dragonfly

Recent years witnessed major improvements in imaging technologies mostly for in vitro analyses of cells; imaging in vivo lagged behind. Despite its prominence as a model, the mouse shows severe limitations for high-resolution imaging, especially when investigating fine object such as therapeutic nanoparticles and the cells with which they interact.
 
Recently, the zebrafish model has emerged as a powerful alternative model to investigate complex biological question in vivo, and this for mainly two reasons:
1) Its small size and transparency at the larvae stage provide excellent conditions for non-invasive live-imaging.
2) Its small size at both larvae and adult stage are also particularly suited for whole-organism investigations.
Together, these two crucial features secure an exceptional preservation of the biological context.
 
Here, I will present the advantages of the zebrafish model for high-resolution imaging and point out how it allowed us to identify a new biological mechanism impacting the fate of intravenously-injected nanoparticles, and to explore the organization of mucosal immunity in fish.
 
Learning Objectives

• Recognize Zebrafish as an excellent model Organism for in vivo imaging.
• Discover how high-resolution microscopy imaging can be used to study nanotechnology and identify new biological mechanisms.
• Illustrate the advantages of 3D multi-fields of view and high-resolution imaging to study the immune system.