Part of the Oxford Instruments Group

Dragonfly Confocal System Upgrades

Dragonfly 200, 300 and 500 microscopes are great instruments and will continue to be supported for many years to come.

By upgrading to deliver capabilities similar to our 600 model, you will increase your productivity and extend the lifetime and usefulness of your system.​

The upgrade will enhance the number of scientific techniques and applications that are possible.​ 

The upgrade process typically takes 1-4 days, and our team of Applications Scientists can help get you up to speed on the new capabilities of the upgraded platform.

Dragonfly 600 Performance Improvements


Borealis Total Internal Reflection Fluorescence (B-TIRF) is Andor’s proprietary TIRF module available on Dragonfly 600.

  • B-TIRF is easy to set up, more robust against optical contamination and consequently more robust to image artefacts. It supports both 60/63x and 100x TIRF objectives.
  • Ideal to increase the signal-to-noise ratio in single-molecule imaging.
  • Image dynamic events at the edge of the coverslip (e.g. membrane fusion events, receptor signaling, and infection dynamics)
  • Compatible with multimode and single mode fibre.
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Comparing conventional TIRF and Borealis TIRF: Left image: single mode TIRF is sensitive to optical contamination resulting in interference fringes and uneven illumination. Right image: the same specimen imaged in B-TIRF with contamination, results in high quality images which reflect the true distribution of fluorescence in the specimen.

High Power Laser Engine

Our new HLE delivers precise, controlled, efficient illumination, and is highly suitable for live-cell imaging and single-molecule imaging. Upgrading to an HLE will enable users to:

  • Speed up spatial transcriptomics and expansion microscopy experiments by decreasing exposure times. 
  • Image sensitive samples for days due to gentle illumination.
  • Break the resolution limit over an order of magnitude (10 nm XY).
  • Get insights into virus-host interaction and virus pathology.
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3D Super-resolution Module

Dragonfly 600 has all the necessary tools for single-molecule localisation microscopy (SMLM).

A single plane image through the 3D Super-resolution module delivers axial information over an ~ 1 μm range with a corresponding resolution down to 30 nm axially.

The 3D super-resolution module, is compatible with all imaging modalities, delivering:

  • Collection of axial SMLM data in widefield mode.
  • Increased the signal-to-noise of the data close to the coverslip.
  • Super-resolution in thicker specimens (~10 μm range) by sequential acquisition of optical sections.
  • This module is ideal for 3D-SMLM applications such as visualizing nuclear pore complexes, the mitochondrial membrane, synapses, the Ultrastructure of Virus Capsid, etc. It brings down the axial resolution to an impressive 30 nm.

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Super-Resolution: SRRF Stream+

SRRF (Super-Resolution Radial Fluctuations) offers a highly effective software-based approach to super-resolution imaging. Our exclusive development, SRRF-Stream+, accelerates processing by delivering real-time super-resolution.​

  • Ideal for live cell imaging
  • Compatible with normal fluorescent labels​
  • Compatible with widefield, TIRF and confocal imaging
  • Compatible with deep imaging and thick samples
  • Available with iXon EMCCD, Sona sCMOS and ZL41 sCMOS cameras
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Widefield & SRRF Steam+


Imaris is the most comprehensive visualization and analysis software for 3D/4D and time-lapse microscopic images. Our AI-powered software enables busy researchers to save time while advancing their research.

  • Comprehensive Analysis Tools - Volumetric, Morphological, Tracking, Filament Tracing
  • Intuitive workflow - from opening data, pre-processing, analysis, interpretation to export
  • Powerful - Imaris handles big microscopy data (Terabyte range) with ease
  • Intelligent - Segment images with machine learning and open analysis results from other software
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Derek Toomre, PhD - Professor of Cell Biology at Yale School of Medicine.

Dragonfly 600 performs at a very high level and its ease of use allows researchers to acquire valuable data at speed. The system really excels for applications that are more demanding, such as high resolution live cell imaging.

Derek Toomre, PhD - Professor of Cell Biology at Yale School of Medicine.

What More Can I Do With My Dragonfly 200?

  • Image 2-6x faster.
  • Gain productivity in spatial biology (e.g. spatial genomics or proteomics).
  • For large fixed sample imaging.
  • Image deeper, enhance your multiplex capabilities, and avoid sample autofluorescence.
  • Add high power NIR (685nm, 730nm & 780nm) lasers.

What More Can I Do With My Dragonfly 500?

  • Image 2-6x faster.
  • Enable new enhanced productivity for spatial biology.
  • Increased productivity for large fixed sample imaging.
  • Add the new Andor HLE to shorten exposures for higher-throughput imaging of large model organisms and tissue samples.
  • Image deeper, enhance your multiplex capabilities, and avoid sample autofluorescence.
  • Add high power NIR (685nm, 730nm & 780nm) lasers.
  • Learn more with a resolution improvement down to 10nm (e.g. cytoskeletal structures, protein-protein relationships, organelles, and nuclear membrane structures).
  • Add B-TIRF for membrane studies and highest contrast imaging of bacteria and yeast, and combine with the new HLE for super-resolution imaging, even in 3D!
  • Add SMLM capabilities for microbiology and ultrastructure of organelles.

Dragonfly Upgrade Path and Options