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Optomask is a C-mount to C-mount relay device with precise aperture control which inserts easily between the microscope output and the camera. Optomask is used in combination with the Crop Mode software feature on iXon EMCCD cameras, resulting in significant increases in Region of Interest (ROI) frame rates. In order to enable Crop Mode it is necessary to accurately optically mask the area of the sensor outside the ROI.
Using the OptoMask, a sub-array can be readily defined through positioning of the masking blades, and a cropped area subsequently matched to this in software.
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The Andor iXon Ultra comes with ‘Optically Centred Crop Mode’, which gives the user the option to break away from the corner tethered requirement of standard Crop Mode and select a number of pre-defined ROIs that are located in the centre of the image field. Optically centring of the ROI makes this mode extremely appealing to a number of microscopy techniques, including ‘pointillism’ live cell super-resolution microscopy.
iXon Ultra/Life 897
| Array Size | ||||
| Binning | 256x256 | 128x128 | 64x64 | 32x32 |
| 1x1 | 111 (174) | 595 (569) | 1433 (1492) | 3532 (3024) |
| 2x2 | 215 (329) | 1085 (1014) | 2433 (2329) | 5325 (4054) |
| 4x4 | 402 (594) | 1802 (1662) | 3577 (3237) | 6579 (5252) |
iXon Ultra/Life 888
| Array Size | ||||
| Binning | 512x512 | 256x256 | 128x128 | 64x64 |
| 1x1 | 93 (78) | 190 (251) | 670 (897) | 2053 (1319) |
| 2x2 | 170 (143) | 350 (426) | 1150 (1019) | 3123 (1646) |
| 4x4 | 291 (245) | 601 (653) | 1772 (1504) | 4109 (1857) |
Above: Significant increases in frame rates may be achieved in Corner Tethered Mode and Optically Centred Crop Mode. Shown at selected ROIs for iXon Ultra 897 (left) and iXon Ultra 888 (right).
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