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Dragonfly

The most complete imaging solution ever... just got better!

NEW Fusion 2.0 provides a workflow from nm’s to mm’s in a single solution

The game-changer in confocal microscopy. With the Andor Dragonfly, you can image at an unrivalled combination of speed, sensitivity, confocality and resolution beyond the diffraction limit. Now available in 200 series and 500 series.

It's more than confocal...

The Dragonfly naturally benefits from Andor EMCCD and sCMOS technologies, with motorized optical zoom, the system delivers industry-leading signal to noise and image fidelity. Applications ranging from single molecule to live cell confocal, TIRFM to whole embryo and thick tissue imaging all benefit from the system’s speed and sensitivity. Dragonfly delivers real-time visualization for rapid specimen evaluation and ClearView-GPU™ deconvolution to maximize resolution and throughput.

  • Instant Confocal
  • Simultaneous multi-color TIRF
  • Laser widefield imaging
  • Single molecule imaging
  • VIS - NIR wavelengths
Specification Sheet
Fast Acquisition, 3D Visualisation, super resolution, low phototoxicity, multi-modal

Fast 2D - 6D imaging

The Dragonfly offers you a sharp insight into the world of your specimen by employing a novel multi-point confocal system, optimized for fast, flexible and sensitive scanning. It extends your sample’s longevity and significantly speeds up data acquisition.

As well as confocal, the system provides further extraordinary control of illumination by offering enhanced widefield and TIRF microscopy, extending the reach from nm to mm and from single molecules to cells, tissues, organoids and embryos.

Traditional Point Scanning Confocal

Dragonfly Confocal Imaging System

Laser Range Confocal Speed Apeture Camera Zoom Illumination Zoom Pinholes Ø
400 - 800 nm 400 fps 22 mm 1x | 1.5x | 2x 1x | 2x | 4x | 6x 40 µm | 25 µm

Dragonfly captures in 16-bit dynamic range, therefore can obtain both low and high signal detail within a single capture.

Alternating Between Low and High Signal in Range
Low and High Signal in Range

Dragonfly Models

Dragonfly is now available in a more compact package, the 200 series, suitable for both inverted and upright microscopes.

The 200 series offers many of the key features that make Dragonfly performance exceptional: Confocal speeds of up to 400 fps; Borealis™ enhanced illumination for a large and flat field of view; ClearView-GPU™ deconvolution; dual-camera acquisition; choice of optical zoom and pinhole size.

For those who might be considering a new or additional confocal, but looking for something that pushes new imaging boundaries. The Dragonfly 200 offers a price-point comparable to traditional point scanning technology.

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Dragonfly 200 Series

Dragonfly 500 offers the full range of imaging modalities and complementary features available on this innovative platform.

Choose this model for a fully scalable imaging range from; sub-cellular protein targets at the cell membrane using TIRF, or deeper in the cell with SRRF-Stream and dSTORM super-resolution; to large scale tissue structure or whole organism anatomy.

Dragonfly 500 should be considered by those whose research projects, or variety of users, cover a wide range of imaging requirements; and for whom the ability to apply several pertinent techniques to the same sample offers a powerful tool for discovery.

Techniques for which you would purchase three independent products, Dragonfly 500 offers in a single cost-effective solution.

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Dragonfly 500 Series

Super-Resolution

The combination of Andor’s Dragonfly and Fusion software provide unparalleled access to super-resolution techniques to image beyond the diffraction limit of optical microscopy. Expand your research from single molecules to whole organisms. All on one system! Choose from our latest camera-based super-resolution technique, SRRF-Stream; iterative ClearView-GPU™ deconvolution or the localisation technique known as dSTORM. Each delivers a different and unique view into the nanometer world.  Choose your technique according to your experimental needs.

Deconvolution

Deconvolution is an invaluable tool for revealing the subtle details of a 3D data set that might otherwise go unnoticed, even in a good quality confocal stack. Break the resolution limit and attain  a lateral resolution down to 140nm and axial resolution to 250nm.

ClearView-GPU™ is an integral part of the system to enhance the finest features of your image. Up to 50x faster than CPU methods, apply ClearView while acquiring images for immediate visualisation and comparison with the original data!

Use deconvolution for the highest 3D resolution, especially powerful as the only super-resolution tool that can be applied to high-speed imaging such as live-cell and multi-dimensional imaging.

Request Pricing Learn More About Clearview-GPU™
Widefield Deconvolution - Dragonfly

SRRF-Stream

Developed in close collaboration with Dr Ricardo Henriques, University College London (UCL), SRRF-Stream offers real-time camera-based super-resolution for live cell imaging across large fields of view, such as those offered by Dragonfly.

SRRF-Stream can be applied to regular fluorescence probes down to a resolution of 50nm and does not require any special sample preparation. Consequently SRRF-Stream can be used on live samples, as well as fixed, and can capture up to 1 frame per second (512x512) ideal for the likes of cytoskeletal dynamics and organelle structure.

Confocal SRRF-Stream - Dragonfly

Deep super-resolution imaging with Confocal SRRF-Stream

Depth colour coded projection of neurons in a 500um thick  spinal cord prepared using the RapidClear method (Sunjin Labs). Volume captured to 100um deep.  Zoomed region demonstrates increased resolution by the clarity of dendritic spines.

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dSTORM

Direct Stochastic Optical Reconstruction Microscopy, or dSTORM, is a single molecule localisation technique that requires high illumination power to drive fluorophores into a dark state. By collecting a large number of frames of different fluorophores within a region, a high-resolution image can be built up.

The Dragonfly 500 series provides the high illumination powers and an optional 3D lens to perform dSTORM acquisitions in 2D and 3D. The dynamic nature of post-processing algorithm developments means that, rather than restrict analysis to Andor software, users are free to select any of their preferred platforms for analysis. Choose to use capture dSTORM image sets when you require resolutions down 20nm.

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Widefield dSTORM - Dragonfly

Why Your Next Confocal Should Be Dragonfly

The Dragonfly naturally benefits from Andor EMCCD and sCMOS technologies, with motorized optical zoom, the system delivers industry-leading signal to noise and image fidelity. Applications ranging from single molecule to live cell confocal, TIRFM to whole embryo and thick tissue imaging all benefit from the system’s speed and sensitivity. Dragonfly delivers real-time visualization for rapid specimen evaluation and GPU-accelerated deconvolution to maximize resolution and throughput.

Discover how Dragonfly excels in:

Click here to enter your details and download the whitepaper

Bacteria to organoids: an imaging platform for all

Confocal, widefield and TIRFM modes for your imaging experiment!

With three modalities at your command, you can explore the in vitro and in vivo biology of many different specimens, selecting the best model organism and modality to suit your needs. Imaging everything from organoids and deep neural structures along the z-dimension to yeast, bacteria and single molecules requires flexibility of imaging mode, magnification and illumination. This level of control preserves specimens and trades speed and longevity in dynamic and large scale imaging tasks.

Please select your preferred illumination method to see how Dragonfly meets your requirements. 

Multi-Point Confocal

At the heart of the Dragonfly Imaging Platform is a newly designed microlens confocal scanner, tightly integrated with our highest sensitivity cameras and presented via our new imaging software, Fusion. The result is speeds of 10 to 20 times faster than a traditional confocal, leaving you with no more waiting for an image to be built up point-by-point and line-by-line. Dragonfly offers the following major benefits:

  • Minimal phototoxicity and photobleaching - ideal for live or delicate specimens.
  • High speeds for imaging fast dynamic events, or high throughput.
  • Large uniform field of view for larger specimens or montage stitching.
multi-point-confocal
multi-point-confocal

Widefield

Confocal is not always the best imaging mode, some samples simply work better with epifluorescence e.g. yeast and other thin specimens. Dragonfly delivers high-contrast high-resolution images using laser widefield and ClearView-GPU™ deconvolution in Fusion. ClearView-GPU™ is CUDA-GPU accelerated and runs 10 to 20 times faster than conventional solutions.

  • Minimal phototoxicity and photobleaching - ideal for live or delicate specimens.
  • Maximize emission bandwidth for high S/N ratio with minimal exposure.
  • ClearView-GPU™ deconvolution delivers clear, sharp images fast.
widefield
widefield

TIRF

Our novel, patent-pending, design offers chromatically corrected TIRF illumination for multi-colour simultaneous imaging at the same penetration depth, ensuring accurate spatial information for detailed interpretation of your results. You can control the critical angle for TIRF and penetration depth into the specimen, or choose to operate in oblique or HILO mode to capture weaker signals at better S/N ratio.

  • Adjust TIRF angle according to specimen configuration.
  • Match penetration depth of two labelled targets. Ideal for intra or extra-cellular imaging.
  • HILO mode for rapid and deeper imaging of the cell, probing its membrane's dynamics.
tirf
tirf
Contact Our Application Specialists

Optical excellence for all experiments

Andor Dragonfly features multiple innovative optical solutions to create the best possible combination of light uniformity, throughput, resolution and power in a confocal microscopy system.

Borealis Uniform Illumination

Uniformity of illumination plays a significant role in obtaining seamless image tiling and more accurate cross-field analysis.

With Borealis, and its unique Perfect Illumination Delivery™ system, you can ensure that the edges and corners of your sample never suffer from non-uniformities.

Micro-lens disk
Micro-lens disk with field lens
Micro-lens disk lens with field lens and Borealis

Magnification

A unique, user-defined, 3-positon zoom in the camera port makes it possible to match the camera pixel size to an objective lens.

This ensures that the Nyquist sampling criterion is met and the specimen is sufficiently oversampled for the best possible spatial resolution.

Magnification at 1x, 1.5x and 2x

Power Density

For certain applications, such as Ca2+ imaging or localization microscopy, a higher power density per unit area may be useful.

In these circumstances, obtaining a high temporal resolution is paramount, while photo-bleaching is not critically endangering the sample during such a time lapse.

Power Density at 1.0, 4.0, 16 and 36

Fusion Stitcher - Big Data Capable

Fusion Stitcher was developed to handle the large multi-dimensional  image files Dragonfly generates.  Start stitching whilst your montage is being captured, so saving valuable time.  Stitch faster in 3D than other solutions can handle in 2D.

We offer the perfect solution for developmental models such as Zebrafish and C. elegans or mapping protein localisation in large areas of tissue such as brain and kidney. The speed of Dragonfly combined with the integrated Fusion Stitcher will save days of valuable time to you can submit that paper ahead of the game!

Large area super-resolution imaging with SRRF-Stream & Stitcher

Combine SRRF-Stream™ and  Stitcher™ for the highest resolution imaging of target samples larger than the field of view of your objective.

Experiment and data management

Fusion is the result of over 100 person-years of shared imaging and development experience, making it easy to design an experiment, choose necessary devices and configure optimal data acquisition protocols. It simplifies the control of the Dragonfly system, translating imaging modes into software states and fluorophores into channels to help you build protocols in just a few mouse clicks.

Integrating deconvolution is simple, tasking the GPU with the associated calculations so that acquisition continues unaffected: a big time saver! Results are delivered with your data, all in one software package. Real-time 3D visualization, split channel view and MIP are fully configurable during acquisition to let you view the data in a mode which best suits your experiment.

Real-Time Rendering

Real-time rendering in Fusion allows for an on-going verification of the quality of your experiment and the status of your sample.

Having a near-instantaneous feedback on the 3D properties of the specimen is critical for evaluation of experimental parameters including signal to noise, illumination power and sample viability, its position in 3D and in relation to its cellular or tissue neighbours. Render quality and speed can be rapidly adjusted following any change in your experiment and can be easily accessed in the Fusion software control panel.

Contact our Software Specialists
 

Protocol Manager

Protocol manager puts you in control of all the tools necessary to create acquisition sequences. It will guide you through the necessary steps of your imaging experiment to produce the most insightful and data-laden image stack.

Add channels from the desired imaging mode and tune the Protocol to your needs, before saving for re-use, per Windows user, along with channel settings and imaging modes. With Fusion software you can plan your experiment in advance, placing the system in an advanced state of readiness, able to begin imaging as soon as you give the go command.

Contact our Software Specialists
 

Image Processing

Fusion’s ClearView-GPU™ deconvolution processing is up to 50x faster than CPU-based methods. There’s no longer a reason not to deconvolve every image and bring out the subtle details that otherwise go unnoticed, even in a good quality confocal stack.

Fusion provides automated tools to combine neighbouring images into large area datasets in 2D and 3D. This is often known as tiling, or montage. Dragonfly’s large field of view and illumination quality provided by Borealis, combined with Fusion’s image processing tools allow large samples to be captured in exquisite detail.

Contact our Software Specialists
 

Simplify experimental workflow

Your Experiemnt -> Data Capture -> Data Visualisation -> Data Analysis

Central to the Dragonfly concept is the goal of maximizing throughput, which is achieved by optimizing both imaging performance and data flow. Fusion and Imaris provide seamless transitions from imaging to deconvolution, visualization to analysis. As a result you spend more time collecting high quality data and less time pre-processing and transferring between systems.

Speed Hypothesis Testing

Hypothesis testing is a fundamental part of the scientific method. The familiar cycle of data creation and evaluation are delivered by the Dragonfly workflow, supporting demanding experimental regimes. Extended observations, large fields of view and high quality data provide the input for powerful statistical analysis and data mining.

Analyse, interpret, assess, reiterate

All the necessary functionality for data management, visualization, analysis, segmentation and interpretation of 3D and 4D microscopy datasets with Imaris

Visualize Complex Data

Welcome to a new era of data interpretation, plotting and presentation. ImarisVantage enables you to compare and contrast experimental groups by visualizing your image data in five dimensions as uni- or multi-variate scatterplots. Along with the use of box + whisker plots Vantage will help you interpret intrinsically complex and dynamic phenomena.

ImarisVantage allows researchers to dissect their multi-dimensional, multi-object images by creating a series of fully customizable plots for better understanding of hidden relationships and associations between calculated measurements, objects or groups of objects.

Contact our applications specialists
 

Trace Filaments

FilamentTracer is the most advanced software product for the automatic detection of neurons (dendritic trees, axons and spines), microtubules, and other filament-like structures in 2D, 3D and 4D.

When combined with ImarisTrack, detection of temporal changes in length and volume of developing spines and dendrites will help researchers understand alterations caused by developmental and environmental changes.

Contact Our Application Specialists
Trace Filaments

Analyse and Understand Cell Fate

ImarisLineage is the new module which builds on the functionality of the powerful and widely used ImarisTrack. ImarisLineage provides tools for accurate automated or manual tracking of dividing cells in 2D/3D time series.

ImarisLineage enables creation of an interactive lineage tree where the cell fate can be traced from one progenitor cell to the final generation.

Contact Our Application Specialists
Analyse and Understand Cell Fate

Additional Resources

The Andor Learning Center hosts a wide range of tutorial videos, technical articles and webinars to guide you through the range of products for all your imaging needs. We have provided some links below which will get you started on some of our most recent uploads.

Customer Publications