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Optosplit III

The Optosplit III, a 3-way image splitter is a simple device for dividing an image into one, two or three separate, spatially equivalent components which can be displayed side by side on a camera sensor, enabling a single camera to record images simultaneously at one, two or three different optical wavelengths.

  • Variable internal path separation
  • Dichroic mirror and emission filters mounted in a readily interchangeable cube
  • Variable and locking rectangular diaphragm aperture
  • Simple and precise controls for image registration


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The Optosplit III has been designed as a convenient, inexpensive solution for simultaneous imaging. Splitting is usually performed on the basis of wavelength or polarization, allowing applications where there is a requirement for simultaneous or high speed acquisition of multiple emission bands or polarization states. The simultaneous acquisition of up to three images offers a major benefit over manual or electronic filter changers, as there is no longer a need to pause acquisition while the filter position is changed. This allows your camera to be operated at the very fastest capture rates it is capable of achieving.

The Optosplit III is usually supplied with unity magnification and fitted with a rectangular aperture to define the ROI. It includes controls to allow up to three images to be positioned accurately and conveniently within the camera frame. Device drivers are included in several commercial imaging packages to assist registration and to allow real-time and off-line ratioing or image overlays. Whilst optimized for coupling to a scientific microscope, the Optosplit III can also be used with camera lenses or any other system of lenses that produce an image plane of suitable size.

Single camera - Cost effective - only one camera required

Variable internal path separation - Minimizing the introduction of aberrations

Dichroic mirror and emission filters mounted in a readily interchangeable cube - Exchange filter sets both easily and quickly. Some competing products have factory fitted filters

Variable and locking rectangular diaphragm aperture for defining field size - Define the ROI both horizontally and vertically and set the images to the optimum size for the camera sensor

Compact design with integral C-mount input and output ports - Integral C-mounts allow it to be easily attached to a wide variety of standard microscopes and CCD cameras

Simple and precise controls for image registration - Split images can be accurately and easily centred in the desired field of view, and pixels aligned with respect to each other

Interchangeable filter/dichroic holders for triple, dual and single wavelength imaging - Flexibility to use multiple wavelengths by simply changing filter and re-sizing the defined field

Aperture diaphragms to balance signal levels if appropriate - Acts as an adjustable neutral density filter, which can be more convenient than using neutral density filters

Rotating filter mount for polarization studies - Accurately orientates the emission polarization to maximize the contrast

Upgrades/Accessories

TR-OPTS-F00 - Empty filter cube for Optosplit II/III

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