Camera based Super Resolution (SRRF-Stream+)

Certain Andor cameras including Sona have been developed to enable super-resolution imaging to be achieved using a normal microscope. This technology is called SRRF-Stream+. It is a cell friendly and easy to use approach to achieving sub-diffraction limited resolutions. It is based on the SRRF (Super Resolution Radial Fluctuations) method of Gustafsson et al (2016). The benefits of this camera and software method are:

• Can use normal fluorophores and labelling protocols

• Use low illumination intensities e.g. LED illumination

• No need for specialised microscope: an existing microscope may be used with a suitable graphics card and compatible Andor camera

This makes it not only a cell-friendly way to perform super resolution imaging, but also makes high resolution imaging accessible a much wider range of researchers. There is little change to the existing imaging workflow. Resolutions down to ~100 nm may be obtained by this method depending on the specific microscope and labelling.

For full information on camera based super-based super resolution and camera compatibility please refer to SRRF-Stream+ Super Resolution.

If you have purchased a SRRF-Stream+ license for your camera please see our support documentation to get started:

• SRRF-Stream+ Quick Start Guide

• SRRF-Stream+ User Guide