Multimodal biological electron microscopy and implications for sample preparation

With multimodal microscopy, we combine different microscopy modalities to visualise the same sample. By imaging the same sample with different methods, we can retrieve structural, physical, and chemical information.

Biological sample preparation is usually tailored towards optimal conditions for a selected imaging modality. With multimodal approaches, it is crucial to consider a flexible specimen preparation to avoid any critical compromise. When electron microscopy is one of the methods of choice, we must prepare conductive and stable samples under the electron beam. Fixation, contrasting, sample topography and coating are going to be discussed during the presentation.

This webinar will cover what factors to consider when preparing biological samples for scanning electron microscopy (SEM) combined with Energy Dispersive X-ray spectrometry (EDS), Raman, and Backscattered Electron and X-ray (BEX) imaging.

Join us and discover:

• How and why to choose the preparation methods that are compatible with multiple imaging workflows
• How to enhance contrast with different staining methods
• What are the benefits of using a multimodal SEM imaging workflow
• Which analytical multimodal SEM workflows to choose for your application